Regioselective Galactosylation of Floxuridine Catalyzed by β-Galactosidase from Bovine Liver in Co-solvent Systems

doi:10.3724/SP.J.1088.2011.01150

Abstract

Abstract: The influence of organic solvents and their contents on the regioselective galactosylation of floxuridine catalyzed by β-galactosidase from bovine liver was investigated in co-solvent systems. It was revealed that the enzyme was unstable and inactivated substantially in organic solvent (10%, v/v)-containing systems, and the detrimental impacts of organic solvents on the enzyme could be relieved remarkably by decreasing their contents. Nevertheless, the addition of organic solvents exerted no influence on the regioselectivity of the enzymatic glycosylation, which retained >99%. The catalytic performance of β-galactosidase from bovine liver correlated well with the dielectric constants of organic solvents in co-solvent systems, among which the enzyme displayed the highest activity in dimethyl sulfoxide (DMSO)-containing system. The yield of desired product reached 71% when the DMSO content was less than 5% (v/v) in the medium.

Key words: β-galactosidase, glycosylation, regioselectivity, floxuridine, organic solvent

YE Min, LIU Qiu-Ping, LI Ning, ZONG Min-Hua. Regioselective Galactosylation of Floxuridine Catalyzed by β-Galactosidase from Bovine Liver in Co-solvent Systems[J]. Chinese Journal of Catalysis, 2011, 32(6): 1063-1068.

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