Preparation of Immobilized Lipase through Combination of Cross-Linked Enzyme Aggregates and Biomimetic Silicification

doi:10.3724/SP.J.1088.2012.11055

Chinese Journal of Catalysis ›› 2012, Vol. 33 ›› Issue (5): 857-862.DOI: 10.3724/SP.J.1088.2012.11055

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Preparation of Immobilized Lipase through Combination of Cross-Linked Enzyme Aggregates and Biomimetic Silicification

JIANG Yanjun1,2, WANG Qi1, WANG Wenqin1, ZHOU Liya1, GAO Jing1,*

1School of Chemical Engineering Hebei University of Technology, Tianjin 300130, China; 2National Key Laboratory of biochemical Engineering Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China

Received:2011-11-21 Revised:2012-01-10 Online:2012-05-09 Published:2012-05-09

Abstract

Abstract: The hybrid biocatalyst was prepared by the combination of cross-linked enzyme aggregates (CLEAs) and biomimetic silicification, where the lipase Candida sp. 99-125 was used as model enzyme. The preparation conditions of lipase-CLEAs were optimized and the resulting activity of 771 U/g was obtained. Under the optimum conditions, 75% of the activity recovery was obtained. P/CLEAs was prepared by coprecipitation of lipase with polyethylene-imine (PEI), and the resulting activity was 897 U/g. The activity recovery of P/CLEAs was about 88%. The silica coating was formed on the outside surface of P/CLEAs via the induction of PEI. The resulting Coated-CLEAs showed high reusability. The stability of the Coated-CLEAs, including the resistance to trypsin and organic solvents, was also significantly improved.

Key words: cross-linked enzyme aggregate, biomimetic silicification, lipase Candida sp. 99-125, genipin, immobilized enzyme

JIANG Yan-Jun, WANG Qi, WANG Wen-Qin, ZHOU Li-Ya, GAO Jing. Preparation of Immobilized Lipase through Combination of Cross-Linked Enzyme Aggregates and Biomimetic Silicification[J]. Chinese Journal of Catalysis, 2012, 33(5): 857-862.

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References

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Preparation of Immobilized Lipase through Combination of Cross-Linked Enzyme Aggregates and Biomimetic Silicification

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