界面活化的溶胶凝胶包埋Candida rugosa脂肪酶催化合成维生素E琥珀酸酯

doi:10.1016/S1872-2067(12)60628-7

催化学报 ›› 2013, Vol. 34 ›› Issue (8): 1608-1616.DOI: 10.1016/S1872-2067(12)60628-7

界面活化的溶胶凝胶包埋Candida rugosa脂肪酶催化合成维生素E琥珀酸酯

胡燚^a,b, 蒋相军^a, 吴素文^a, 江凌^a,b, 黄和^a,b

a 南京工业大学生物与制药工程学院, 江苏南京210009;
b 南京工业大学材料化学工程国家重点实验室, 江苏南京210009

收稿日期:2013-04-22 修回日期:2013-05-28 出版日期:2013-08-16 发布日期:2013-07-30
通讯作者: 黄和
基金资助:
国家杰出青年科学基金(21225626);国家自然科学基金(20906049);国家高技术研究发展计划(863计划,2011AA02A209).

Synthesis of vitamin E succinate by interfacial activated Candida rugosa lipase encapsulated in sol-gel materials

Yi Hu^a,b, Xiangjun Jiang^a, Suwen Wu^a, Ling Jiang^a,b, He Huang^a,b

a College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, Jiangsu, China;
b State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology, Nanjing 210009, Jiangsu, China

Received:2013-04-22 Revised:2013-05-28 Online:2013-08-16 Published:2013-07-30
Supported by:
This work was supported by the National Science Foundation for Distinguished Young Scholars of China (21225626), the National Natural Science Foundation of China (20906049), and the High Technology Research and Development Program of China (863 Program, 2011AA02A209).

摘要/Abstract

摘要： 采用界面活化的溶胶凝胶包埋Candida rugosa脂肪酶(CRL)催化合成了维生素E琥珀酸酯.考察了影响溶胶凝胶包埋固定化CRL的因素,获得的最佳固定化条件为:丙基三甲氧基硅烷/正硅酸四乙酯摩尔比为1/1,水与硅烷前体摩尔比为15,酶的添加量为0.5mg/ml,PEG400的添加量为12μl/ml溶胶. 溶胶凝胶包埋的CRL在50℃,18h后其活性仍然保持了70.58%,是游离酶的2.6倍,且稳定性得到了明显的改善.基于CRL的界面特性,采用五种表面活性剂对其进行界面活化.结果表明,采用橄榄油活化的溶胶凝胶包埋的CRL合成维生素E琥珀酸酯的酯化活力最高,相比原酶和未界面活化的溶胶凝胶包埋酶分别提高了6.7和1.43倍.

关键词: 溶胶凝胶, 界面活化, Candida rugosa脂肪酶, 维生素E琥珀酸酯

Abstract: Vitamin E succinate was synthesized by interfacial activated Candida rugosa lipase (CRL) encapsulated in sol-gel materials. The effects of various immobilization parameters were investigated. The optimum conditions were found to consist of n-propyltrimethoxysilane/tetraethoxysilane molar ratio=1/1, water/silane molar ratio (R value)=20, lipase loading=0.5 mg/ml sol, and PEG400 loading=12 μl/ml sol. Compared with free enzymes, sol-gel encapsulated enzymes kept its activity of 70.58% and achieved 2.6-fold increased stability after 18 h incubation in phosphate buffer (0.025 mol/L, pH=7.0) at 50℃. Based on the interfacial characteristics of CRL, five kinds of surfactants were used for activation. The results demonstrate that olive oil was the most effective in activating CRL. The esterification activity of vitamin E succinate synthesis by interfacial activated CRL encapsulated in sol-gel materials reached up to 6.7-fold and 1.43-fold that of free enzyme and non-interfacial activated enzyme, respectively.

Key words: Sol-gel, Interfacial activation, Candida rugosa lipase, Vitamin E succinate

胡燚, 蒋相军, 吴素文, 江凌, 黄和. 界面活化的溶胶凝胶包埋Candida rugosa脂肪酶催化合成维生素E琥珀酸酯[J]. 催化学报, 2013, 34(8): 1608-1616.

Yi Hu, Xiangjun Jiang, Suwen Wu, Ling Jiang, He Huang. Synthesis of vitamin E succinate by interfacial activated Candida rugosa lipase encapsulated in sol-gel materials[J]. Chinese Journal of Catalysis, 2013, 34(8): 1608-1616.

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参考文献

[1] Bornscheuer U T, Huisman G W, Kazlauskas R J, Lutz S, Moore J C, Robins K. Nature, 2012, 485: 185

[2] Hu Y, Liu W M, Zou B, Tang S S, Huang H. Prog Chem (胡燚,刘维明,邹彬,唐苏苏,黄和. 化学进展), 2010, 22: 1656

[3] Reetz M T, Zonta A, Simpelkamp J. Biotechnol Bioeng, 1996, 49: 527

[4] Gill I, Ballesteros A. Trends Biotechnol, 2000, 18: 282

[5] Nguyen D T, Smit M, Dunn B, Zink J I. Chem Mater, 2002, 14: 4300

[6] Noureddini H, Gao X, Joshi S, Wagner P. J Am Oil Chem Soc, 2002, 79: 33

[7] Maruyama T, Nakajima M, Uchikawa S, Nabetani H, Furusaki S, Seki M. J Am Oil Chem Soc, 2000, 77: 1121

[8] Foresti M L, Alimenti G A, Ferreir M L. Enzyme Microb Technol, 2005, 36: 338

[9] Yilmaz E. World J Microbiol Biotechnol, 2002, 18: 37

[10] Prasad K N, Kumar B, Yan X D, Hanson A J, Cole W C. J Am Col Nutrition, 2003, 22: 108

[11] Quin J, Engle D, Litwiller A, Peralta E, Grasch A, Boley T, Hazelrigg S. J Surgical Res, 2005, 127: 139

[12] Zheng GW, Xu J H. Curr Opin Biotechno, 2011, 22: 784

[13] Kapoor M, Gupta M N. Process Biochem, 2012, 47: 555

[14] Tang S S, Hu Y, Yu D H, Zou B, Jiang L. Chin J Catal (唐苏苏,胡燚,余定华,邹彬,江凌. 催化学报),2012, 33: 1473

[15] Ding Y, Huang H, Hu Y. Chin J Org Chem (丁雁,黄和,胡燚. 有机化学), 2013, 33: 905

[16] Torres P, Reyes-Duarte D, López-Cortés N, Ferrer M, Ballesteros A, Plou F J. Process Biochem, 2008, 43: 145

[17] Yin C H, Zhang C, Gao M. Chin J Chem Eng, 2011, 19: 135

[18] Jiang X J, Hu Y, Jiang L, Gong J H, Huang H. Chem Res Chin Univ, 2013, 29: 223

[19] Bradford M M. Anal Biochem, 1976, 72: 248

[20] Cao X W, Yang J K, Shu L, Yu B Q, Yan Y J. Process Biochem, 2009, 44: 177

[21] Chen J P, Lin W S. Enzyme Microb Technol, 2003, 32: 801

[22] Menaa B, Herrero M, Rives V, Lavrenko M, Eggers D K. Biomaterials, 2008, 29: 2710

[23] Yang G, Wu J P, Xu G, Yang L. Bioresour Technol, 2009, 100: 4311

[24] Soares C M F, dos Santos O A, De Castro H F, De Moraes F F, Zanin G M. Appl Biochem Biotechnol, 2004, 113: 307

[25] Gottfried D S, Kagan A, Hoffman B M, Friedman J M. J Phy Chem B, 1999, 103: 2803

[26] Van Tilbeurgh H, Egloff M P, Martinez C, Rugani N, Verger R, Cambillau C. Nature, 1993, 362: 814

[27] Miled N, Bussetta C, De Caro A, Rivière M, Berti L, Canaan S. Comp Biochem Phys B, 2003, 136: 131

[28] Yang J K, Liu LY, Cao X W. Enzyme Microb Technol, 2010, 46: 257

界面活化的溶胶凝胶包埋Candida rugosa脂肪酶催化合成维生素E琥珀酸酯

Synthesis of vitamin E succinate by interfacial activated Candida rugosa lipase encapsulated in sol-gel materials

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