仿生制备有机-无机复合微囊固定化葡萄糖氧化酶

doi:10.1016/S1872-2067(12)60635-4

催化学报 ›› 2013, Vol. 34 ›› Issue (8): 1627-1633.DOI: 10.1016/S1872-2067(12)60635-4

• 研究论文 • 上一篇

仿生制备有机-无机复合微囊固定化葡萄糖氧化酶

辛茜^a, 姜艳军^a,b, 高静^a, 周丽亚^a, 马丽^a, 贺莹^a, 贾霏^a

a 河北工业大学化工学院, 天津300130;
b 中国科学院过程研究所生化工程国家重点实验室, 北京100190

收稿日期:2013-03-25 修回日期:2013-06-19 出版日期:2013-08-16 发布日期:2013-07-30
通讯作者: 姜艳军,高静
基金资助:
国家自然科学基金(21006020, 21276060, 21276062); 河北省应用基础研究计划重点基础研究项目(11965150D); 天津自然科学基金(13JCYBJC18500); 生化工程国家重点实验室开放课题.

Biomimetic preparation of organic-inorganic composite microcapsules for glucose oxidase immobilization

Qian Xin^a, Yanjun Jiang^a,b, Jing Gao^a, Liya Zhou^a, Li Ma^a, Ying He^a, Fei Jia^a

a School of Chemical Engineering, Hebei University of Technology, Tianjin 300130, China;
b National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China

Received:2013-03-25 Revised:2013-06-19 Online:2013-08-16 Published:2013-07-30
Supported by:
This work was supported by the National Natural Science Foundation of China (21006020, 21276060, 21276062), the Application Basic Research Plan Key Basic Research Project of Hebei Province (11965150D), the Natural Science Foundation of Tianjin (13JCYBJC18500), and the Open Funding Project of the National Key Laboratory of Biochemical Engineering (China).

摘要/Abstract

摘要： 将层层自组装技术与仿生矿化技术相结合,由聚苯乙烯磺酸钠、聚二甲基二烯丙基氯化铵和二氧化硅成功制备(聚苯乙烯磺酸钠-聚二甲基二烯丙基氯化铵)₂-二氧化硅复合微囊.采用扫描电子显微镜、红外光谱和热重对微囊的形貌和化学结构进行了表征.以该复合微囊作为理想载体固定化葡萄糖氧化酶.结果表明,固定于复合微囊中的葡萄糖氧化酶的热稳定性、pH稳定性、操作稳定性得到了提高;在最适条件下,复合微囊固定化葡萄糖氧化酶的酶活回收率为72.85%,米氏常数是游离葡萄糖氧化酶的2.21倍.复合微囊在化学/生物催化、药物/基因传递系统和生物传感器应用方面具有一定的潜能.

关键词: 复合微囊, 层层自组装, 仿生矿化, 葡萄糖氧化酶

Abstract: Organic-inorganic composite microcapsules made of sodium poly(styrenesulfonate) (PSS), poly(dimethyl diallyl ammonium chloride) (PDADMAC), and biomimetic silica were successfully fabricated via a combination of layer-by-layer self-assembly and biomimetic mineralization. The morphologies and chemical compositions of the microcapsules (denoted by (PSS/PDADMAC)₂-SiO₂) were characterized using scanning electron microscopy, Fourier-transform infrared spectroscopy, and thermogravimetric analysis. The composite microcapsules were used as carrier for the immobilization of glucose oxidase (GOD). GOD encapsulated in (PSS/PDADMAC)₂-SiO₂ (denoted by GOD@(PSS/PDADMAC)₂-SiO₂) displayed improved thermal, pH, and operational stabilities. The activity recovery of the immobilized GOD reached 72.85% under the optimized conditions. GOD@(PSS/PDADMAC)₂-SiO₂ showed a 2.21-fold increase in the Michaelis constant (K_m) compared with free GOD. The composite microcapsules have the potential for a range of applications in chemical/biological catalysis and drug/gene delivery systems, and as biosensors.

Key words: Composite microcapsule, Layer-by-layer assembly, Biomimetic mineralization, Glucose oxidase

辛茜, 姜艳军, 高静, 周丽亚, 马丽, 贺莹, 贾霏. 仿生制备有机-无机复合微囊固定化葡萄糖氧化酶[J]. 催化学报, 2013, 34(8): 1627-1633.

Qian Xin, Yanjun Jiang, Jing Gao, Liya Zhou, Li Ma, Ying He, Fei Jia. Biomimetic preparation of organic-inorganic composite microcapsules for glucose oxidase immobilization[J]. Chinese Journal of Catalysis, 2013, 34(8): 1627-1633.

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仿生制备有机-无机复合微囊固定化葡萄糖氧化酶

Biomimetic preparation of organic-inorganic composite microcapsules for glucose oxidase immobilization

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