Biomimetic preparation of organic-inorganic composite microcapsules for glucose oxidase immobilization

doi:10.1016/S1872-2067(12)60635-4

Chinese Journal of Catalysis ›› 2013, Vol. 34 ›› Issue (8): 1627-1633.DOI: 10.1016/S1872-2067(12)60635-4

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Biomimetic preparation of organic-inorganic composite microcapsules for glucose oxidase immobilization

Qian Xin^a, Yanjun Jiang^a,b, Jing Gao^a, Liya Zhou^a, Li Ma^a, Ying He^a, Fei Jia^a

a School of Chemical Engineering, Hebei University of Technology, Tianjin 300130, China;
b National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China

Received:2013-03-25 Revised:2013-06-19 Online:2013-08-16 Published:2013-07-30
Supported by:
This work was supported by the National Natural Science Foundation of China (21006020, 21276060, 21276062), the Application Basic Research Plan Key Basic Research Project of Hebei Province (11965150D), the Natural Science Foundation of Tianjin (13JCYBJC18500), and the Open Funding Project of the National Key Laboratory of Biochemical Engineering (China).

Abstract

Abstract: Organic-inorganic composite microcapsules made of sodium poly(styrenesulfonate) (PSS), poly(dimethyl diallyl ammonium chloride) (PDADMAC), and biomimetic silica were successfully fabricated via a combination of layer-by-layer self-assembly and biomimetic mineralization. The morphologies and chemical compositions of the microcapsules (denoted by (PSS/PDADMAC)₂-SiO₂) were characterized using scanning electron microscopy, Fourier-transform infrared spectroscopy, and thermogravimetric analysis. The composite microcapsules were used as carrier for the immobilization of glucose oxidase (GOD). GOD encapsulated in (PSS/PDADMAC)₂-SiO₂ (denoted by GOD@(PSS/PDADMAC)₂-SiO₂) displayed improved thermal, pH, and operational stabilities. The activity recovery of the immobilized GOD reached 72.85% under the optimized conditions. GOD@(PSS/PDADMAC)₂-SiO₂ showed a 2.21-fold increase in the Michaelis constant (K_m) compared with free GOD. The composite microcapsules have the potential for a range of applications in chemical/biological catalysis and drug/gene delivery systems, and as biosensors.

Key words: Composite microcapsule, Layer-by-layer assembly, Biomimetic mineralization, Glucose oxidase

Qian Xin, Yanjun Jiang, Jing Gao, Liya Zhou, Li Ma, Ying He, Fei Jia. Biomimetic preparation of organic-inorganic composite microcapsules for glucose oxidase immobilization[J]. Chinese Journal of Catalysis, 2013, 34(8): 1627-1633.

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Biomimetic preparation of organic-inorganic composite microcapsules for glucose oxidase immobilization

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