Immobilized cellulase on Fe3O4 nanoparticles as a magnetically recoverable biocatalyst for the decomposition of corncob

doi:10.1016/S1872-2067(15)61028-2

Chinese Journal of Catalysis ›› 2016, Vol. 37 ›› Issue (3): 389-397.DOI: 10.1016/S1872-2067(15)61028-2

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Immobilized cellulase on Fe₃O₄ nanoparticles as a magnetically recoverable biocatalyst for the decomposition of corncob

Qikun Zhang, Junqing Kang, Bing Yang, Leizhen Zhao, Zhaosheng Hou, Bo Tang

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan 250014, Shandong, China

Received:2015-12-02 Revised:2015-12-08 Online:2016-02-29 Published:2016-02-29
Contact: Qikun Zhang
Supported by:
This work was supported by the National Basic Research Program of China (973 Program, 2013CB933800), the Natural Science Foundation of Shandong Province (ZR2013EMM004), the Jinan University Independent Innovation Program (201401245), and the College Students, Innovation Training Program of Ministry of Education (201410445074).

Abstract

Abstract:

A magnetically recoverable biocatalyst was successfully prepared through the immobilization of cellulase onto Fe₃O₄ nanoparticles. The magnetic nanoparticles were synthesized by a hydrothermal method in an aqueous system. The support (Fe₃O₄ nanoparticles) was modified with (3-aminopropyl)triethoxysilane, and glutaraldehyde was used as the cross-linker to immobilize the cellulose onto the modified support. Different factors that influence the activity of the immobilized enzyme were investigated. The experimental results indicated that the suitable immobilization temperature and pH are 40 ℃ and 6.0, respectively. The optimal glutaraldehyde concentration is ~2.0 wt%, and the appropriate immobilization time is 4 h. Under these optimal conditions, the activity of the immobilized enzyme could be maintained at 99.1% of that of the free enzyme. Moreover, after 15 cyclic runs, the activity of the immobilized enzyme was maintained at ~91.1%. The prepared biocatalyst was used to decompose corncobs, and the maximum decomposition rate achieved was 61.94%.

Key words: Magnetic nanoparticle, Cellulase, Enzyme immobilization, Corncob, Glutaraldehyde

Qikun Zhang, Junqing Kang, Bing Yang, Leizhen Zhao, Zhaosheng Hou, Bo Tang. Immobilized cellulase on Fe₃O₄ nanoparticles as a magnetically recoverable biocatalyst for the decomposition of corncob[J]. Chinese Journal of Catalysis, 2016, 37(3): 389-397.

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Immobilized cellulase on Fe₃O₄ nanoparticles as a magnetically recoverable biocatalyst for the decomposition of corncob

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