Kinetic study and kinetic parameters of lipase-catalyzed glycerolysis of sardine oil in a homogeneous medium

doi:10.1016/S1872-2067(15)61040-3

Abstract

Abstract:

The production of polyunsaturated fatty acids (PUFAs) concentrates by enzymatic catalysis has gained interest due to their stereospecificity and the milder conditions employed compared to the use of inorganic catalysts. The enzymatic glycerolysis of sardine oil by Lipozyme® 435 to get PUFA concentrates in the forms of di- and monoacylglycerols (DAGs, MAGs) in an optimized amount of tert-butanol as the organic solvent was studied. First, mass transfer limitation of the reaction system was analyzed. The effects of different operating variables such as lipase loading, temperature and feed composition were investigated. A semi-empirical kinetic model based on the reversible elementary reactions of glycerolysis and hydrolysis of the glycerides was employed to correlate the experimental kinetic data. A molar ratio glycerol:oil of 3:1 was the optimum, which produced more than 84 wt% of MAG at 323 K. A comparison with other glycerolysis systems was performed using MAG yield, reaction rate and significance of kinetic parameters.

Key words: Lipase, Glycerolysis, Tert-butanol, Mass transfer, Kinetic model

Ángela García Solaesa, María Teresa Sanz, Sagrario Beltrán, Rodrigo Melgosa. Kinetic study and kinetic parameters of lipase-catalyzed glycerolysis of sardine oil in a homogeneous medium[J]. Chinese Journal of Catalysis, 2016, 37(4): 596-606.

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URL: https://www.cjcatal.com/EN/10.1016/S1872-2067(15)61040-3

https://www.cjcatal.com/EN/Y2016/V37/I4/596

References

[1] E. A. M. De Deckere, O. Korver, P. M. Verschuren, M. B. Katan, Eur. J. Clin. Nutr., 1998, 52, 749-753.
[2] P. M. Kris-Etherton, W. S. Harris, L. J. Appel, Circulation, 2002, 106, 2747-2757.
[3] P. D. Nichols, A. McManus, K. Krail, A. J. Sinclair, M. Miller, Nutrients, 2014, 6, 3727-3733.
[4] E. M. Hernandez, Lipid. Technol., 2014, 26, 103-106.
[5] L. D. Lawson, B. G. Hughes, Biochem. Biophys. Res. Commun., 1988, 152, 328-335.
[6] N. J. Zhong, L. Li, X. B. Xu, L. Z. Cheong, B. Li, S. Q. Hu, X. H. Zhao, J. Am. Oil. Chem. Soc., 2009, 86, 783-789.
[7] U. T. Bornscheuer, Enzyme Microb. Technol., 1995, 17, 578-586.
[8] M. L. Damstrup, T. Jensen, F. V. Sparsø, S. Z. Kiil, A. D. Jensen, X. Xu, J. Am. Oil Chem. Soc., 2005, 82, 559-564.
[9] P. H. L. Moquin, F. Temelli, J. W. King, M. M. Palcic, J. Am. Oil Chem. Soc., 2005, 82, 613-617.
[10] Z. Guo, X. B. Xu, Green Chem., 2006, 8, 54-62.
[11] A. Valério, R. L. Krüger, J. Ninow, F. C. Corazza, D. De Oliveira, J. Vladimir Oliveira, M. L. Corazza, J. Agric. Food Chem., 2009, 57, 8350-8356.
[12] K. G. Fiametti, M. K. Ustra, D. De Oliveira, M. L. Corazza, A. Furigo Jr, J. Vladimir Oliveira, Ultrason. Sonochem., 2012, 19, 440-451.
[13] Á. G. Solaesa, S. L. Bucio, M. T. Sanz, S. Beltrán, S. Rebolleda, Fluid Phase Equilib., 2013, 356, 284-290.
[14] M. L. Damstrup, J. Abildskov, S. Kiil, A. D. Jensen, F. V. Sparsø, X. B. Xu, J. Agric. Food Chem., 2006, 54, 7113-7119.
[15] Á. G. Solaesa, M. T. Sanz, M. Falkeborg, S. Beltrán, Z. Guo, Food Chem., 2016, 190, 960-967.
[16] R. L. Krüger, A. Valério, M. Balen, J. L. Ninow, J. Vladimir Oliveira, D. de Oliveira, M. L. Corazza, Eur. J. Lipid Sci. Technol., 2010, 112, 921-927.
[17] F. Voll, R. L. Krüger, F. de Castilhos, L. Cardozo Filho, V. Cabral, J. Ninow, M. L. Corazza, Biochem. Eng. J., 2011, 56, 107-115.
[18] N. Majid, B. Cheirsilp, Int. J. Food Sci. Technol., 2012, 47, 793-800.
[19] F. K. Zeng, B. Yang, Y. H. Wang, W. F. Wang, Z. X. Ning, L. Li, J. Am. Oil Chem. Soc., 2010, 87, 531-537.
[20] T. Yang, M. Rebsdorf, U. Engelrud, X. B. Xu, J. Agric. Food Chem., 2005, 53, 1475-1481.
[21] T. W. Tan, C. H. Yin, Biochem. Eng. J., 2005, 25, 39-45.
[22] B. Cheirsilp, W. Kaewthong, A. H-Kittikun, Biochem. Eng. J., 2007, 35, 71-80.
[23] Á. G. Solaesa, S. L. Bucio, M. T. Sanz, S. Beltrán, S. Rebolleda, J. Oleo. Sci., 2014, 63, 449-460.
[24] M. T. Sanz, R. Murga, S. Beltrán, J. L. Cabezas, J. Coca, Ind. Eng. Chem. Res., 2002, 41, 512-517.
[25] F. G. Helfferich, Ion Exchange, McGraw-Hill, New York, 1962.
[26] D. M. Chesterfield, P. L. Rogers, E. O. Al-Zaini, A. A .Adesina, Chem. Eng. J., 2012, 207-208, 701-710.
[27] H. P. Dong, Y. J. Wang, Y. G. Zheng, J. Mol. Catal. B, 2010, 66, 90-94.
[28] H. S. Fogler, Elements of Chemical Reaction Engineering, 3rd ed, Prentice-Hall International, Inc., New Jersey, 1999.
[29] R. C. Reid, J. M. Prausnitz, B. E. Poling, The Properties of Gases & Liquids, 4th ed, McGraw-Hill Book Company, New york, 1987.
[30] P. Olivares-Carrillo, J. Quesada-Medina, A. P. de los Ríos, F. J. Hernández-Fernández, Chem. Eng. J., 2014, 241, 418-432.
[31] J. E. Bailey, D. F. Ollis, Biochemical Engineering Fundamentals, Mcgrawhill, New York, 1986.
[32] J. Pleiss, M. Fischer, R. D. Schmid, Chem. Phys. Lipids, 1998, 93, 67-80.
[33] S. L. Bucio, Á. G. Solaesa, M. T. Sanz, R. Melgosa, S. Beltrán, H. Sovová, J. Oleo. Sci., 2015, 64, 431-441.
[34] R. J. Sengwa, V. Khatri, S. Choudhary, S. Sankhla, J. Mol. Liq., 2010, 154, 117-123.
[35] F. V. K. Young, The Chemical & Physical Properties of Crude Fish Oils for Refiners & Hidrogenators, Fish Oil Bulletin, 1986.
[36] F. I. Chowdhury, M. A. Saleh, J. Mol. Liq., 2014, 191, 156-160.
[37] R. Pawongrat, X. Xu, A. H-Kittikun, J. Sci. Food Agric., 2008, 88, 256-262.
[38] R. Pawongrat, X. Xu, A. H-Kittikun, Food Chem., 2007, 104, 251-258.