催化学报

催化学报 ›› 2008, Vol. 29 ›› Issue (10): 1021-1026.

• 研究论文 • 上一篇    下一篇

磁性纳米氮化铝颗粒固定化β-葡萄糖苷酶的性质

潘利华,罗建平,王贵娟,徐学玲,宛雯   

  1. 合肥工业大学生物与食品工程学院生物工程系, 安徽合肥 230009
  • 收稿日期:2008-10-25 出版日期:2008-10-25 发布日期:2008-10-25

Properties of β-Glucosidase Immobilized on Magnetic Nanoparticles of Aluminum Nitride

PAN Lihua, LUO Jianping*, WANG Guijuan, XU Xueling, WAN Wen   

  1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, Anhui, China
  • Received:2008-10-25 Online:2008-10-25 Published:2008-10-25

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摘要： 以戊二醛为交联剂,研究了磁性纳米氮化铝颗粒固定化β-葡萄糖苷酶的条件及固定化酶的结构特征,并考察了固定化酶的某些酶学性质. 结果表明,在4.5 ml磁性纳米氮化铝颗粒悬液(100 mg/ml)中加入0.5 ml戊二醛溶液(2%)超声波分散后,加入5 mlβ-葡萄糖苷酶溶液(50 mg/ml), 于20 ℃, pH 5.0和100 r/min条件下固载3.5 h, 酶蛋白和酶活回收率分别为82.6%和78.4%. 固定化β-葡萄糖苷酶的结构松散,不改变酶的结构特征. 与游离酶相比,固定化酶对对硝基苯基-β-D-葡萄糖苷水解反应的最佳反应温度有所降低,最佳反应pH值有所升高,而米氏常数Km值有所增大,且具有良好的贮存稳定性和操作稳定性,表明磁性纳米氮化铝颗粒适合作为β-葡萄糖苷酶的固定化载体.

关键词: 磁性纳米颗粒, 氮化铝, 葡萄糖苷酶, 固定化, 戊二醛

Abstract: The conditions for β-glucosidase immobilization on magnetic nanoparticles of aluminum nitride were optimized, and the properties of immobilized β-gl ucosidase including structure characterization and catalytic characteristics wer e investigated. The magnetic nanoparticle of aluminum nitride is a good support for the immobilization of β-glucosidase.

Key words: magnetic nanoparticle, aluminum nitride, glucosidase, immobilization, glutaraldehyde