催化学报

催化学报 ›› 2011, Vol. 32 ›› Issue (6): 1035-1039.DOI: 10.3724/SP.J.1088.2011.01236

• 研究论文 • 上一篇    下一篇

酵母静息细胞催化丙酮酸乙酯不对称还原制 (S)-乳酸乙酯

王丹1,2, 张强 2, 李旺 2, 戚南昌 3, 郭春晓 1, 杨志荣 1, 张杰 1,*   

  1. 1四川大学生命科学学院, 四川成都 610065; 2成都医学院生物医学系, 四川成都 610083; 3成都医学院医学检验系, 四川成都 610083
  • 收稿日期:2011-01-05 修回日期:2011-03-03 出版日期:2011-06-21 发布日期:2014-10-31

Asymmetric Reduction of Ethyl Pyruvate Catalyzed by Yeast Resting Cells to (S)-Ethyl Lactate

WANG Dan1,2 , ZHANG Qiang2, LI Wang2, QI Nanchang3, GUO Chunxiao1, YANG Zhirong1, ZHANG Jie1,*   

  1. 1Life Science College, Sichuan University, Chengdu 610065, Sichuan, China; 2Department of Biomedicine, Chengdu Medical College, Chengdu 610083, Sichuan, China; 3Department of Medical Examination, Chengdu Medical College, Chengdu 610083, Sichuan, China
  • Received:2011-01-05 Revised:2011-03-03 Online:2011-06-21 Published:2014-10-31

PDF

900

被引次数

16

摘要： 从污水处理池及其附近土壤中分离到 36 株可将丙酮酸乙酯不对称还原成 (S)-乳酸乙酯的菌株, 经多次复筛, 最终得到一株具有较高催化活性的酵母菌 BTY18-6. 以 BTY18-6 的静息细胞为催化剂, 在水相中进行丙酮酸乙酯不对称还原成 (S)-乳酸乙酯的反应, 并对反应条件进行了优化. 结果表明, 以 2.5% 葡萄糖为辅助底物, 反应体系初始 pH = 6.8, 发酵培养 48 h 的菌体湿度 0.175 g/ml, 丙酮酸乙酯初始浓度 65 mmol/L, 于 32 oC 反应 48 h 的条件下, 丙酮酸乙酯转化率达 95.5%, 产物对映体过量值 (ee 值) 为 92.1%.

关键词: 酵母静息细胞, 生物催化, 丙酮酸乙酯, 不对称还原, (S)-乳酸乙酯

Abstract: Thirty six strains that exhibited the catalytic activity for asymmetric reduction of ethyl pyruvate to (S)-ethyl lactate were isolated from sewage of a chemical factory in Chengdu, China and the neighboring soil. Among them, yeast strain BTY18-6 has high catalytic activity. The asymmetric reduction of ethyl pyruvate to (S)-ethyl lactate catalyzed by BTY18-6 resting cells in an aqueous phase was examined, and the parameters influencing the bio-reduction were optimized. The results showed that, under the optimized conditions of 2.5% glucose as co-substrate, the initial reaction pH = 6.8, the initial cell concentration at 0.175 g/ml (wet mass) cultured for 48 h, the initial ethyl pyruvate concentration at 65 mmol/L, temperature 32 oC, and reaction time 48 h, the ethyl pyruvate conversion reached 95.5% with enantiomeric excess of 92.1%.

Key words: yeast resting cell, biological catalysis, ethyl pyruvate, asymmetric reduction, (S)-ethyl lactate