催化学报

催化学报 ›› 2009, Vol. 30 ›› Issue (1): 48-52.

• 研究论文 • 上一篇    下一篇

固态发酵-水煮耦合炮制牛蒡子提高牛蒡子苷元含量

欧志敏1,郭妨元1,杨根生1,姚善泾2   

  1. 1 浙江工业大学药学院, 浙江杭州 310014; 2 浙江大学生物工程研究所, 浙江杭州 310027
  • 收稿日期:2009-01-25 出版日期:2009-01-25 发布日期:2012-10-17

Enhancement of Arctigenin Content by Processing of Fructus Arctii via Solid-State Fermentation Coupled with Poach

OU Zhimin1, GUO Fangyuan1, YANG Gensheng1*, YAO Shanjing2   

  1. 1 Pharmaceuticals College, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China; 2 Institute of Bioengineering, Zhejiang University, Hangzhou 310027, Zhejiang, China
  • Received:2009-01-25 Online:2009-01-25 Published:2012-10-17

PDF

940

被引次数

10

摘要： 研究了黑曲霉Aspergillus niger ZJUT712菌株固态发酵和水煮工艺相耦合炮制牛蒡子. 结果表明,该工艺提高了牛蒡子中有效成分牛蒡子苷元的含量,从而有利于促进牛蒡子摄入体内迅速起效. 最佳固态发酵炮制条件为: 0.5 g牛蒡子粉、3 g麸皮、2 g甘蔗渣、0.3 g蛋白胨和10 ml Mandels营养液,固液比1∶3.6 g/ml, 初始pH 5.6, 30 ℃发酵7 d. 牛蒡子苷元产率随底物初始浓度的增加而降低. A.niger ZJUT712转化0.174 mmol/L牛蒡子苷的产率可达93.0%. 7 d时间内A.niger ZJUT712水解牛蒡子苷的过程符合Monod方程,反应动力学常数为Vm=0.0837mmol/(L•d), Km=0.16 mmol/L.

关键词: 牛蒡子苷, 牛蒡子苷元, β-葡萄糖苷酶, 中药, 发酵, 黑曲霉Aspergillus niger ZJUT712

Abstract: The arctigenin content in fructus arctii has been increased by solid-state ferm entation coupled with poach. The optimum medium components are 0.5 g fructus ar c tii powder, 3 g bran, 2 g bagasse, 0.3 g peptone, and 10 ml Mandels nutrient fl uid. Solid-to-liquid ratio is 1∶3.6 g/ml. Initial pH of the medium is 5.6. The yield reached 93.0% when 0.174 mmol/L arctiin was hydrolyzed at 30 ℃ for 7 d. In 7 d, the hydrolysis of arctiin is in accordance with the equation of Monod.

Key words: arctiin, arctigenin, β-glucosidase, Chinese medicine, fermentation, Aspergillus niger ZJUT712