脂质体为模板仿生硅化固定葡萄糖氧化酶

doi:10.1016/S1872-2067(11)60519-6

催化学报 ›› 2013, Vol. 34 ›› Issue (4): 741-750.DOI: 10.1016/S1872-2067(11)60519-6

脂质体为模板仿生硅化固定葡萄糖氧化酶

朱亚男^a, 姜艳军^a,b, 高静^a, 周丽亚^a, 贺莹^a, 贾霏^a

a 河北工业大学化工学院, 天津300130;
b 中国科学院过程工程研究所生化工程国家重点实验室, 北京100190

收稿日期:2012-10-30 修回日期:2012-12-24 出版日期:2013-04-23 发布日期:2013-04-24
通讯作者: 姜艳军,高静
基金资助:
国家自然科学基金(21006020,21276060,21276062);河北省自然科学基金(B2010000035,B2011202095);河北省高等学校科学技术研究重点项目(ZD2010118);河北省应用基础研究计划重点基础研究项目(11965150D);生化工程国家重点实验室开放课题.

Immobilization of glucose oxidase in liposome-templated biomimetic silica particles

ZHU Yanan^a, JIANG Yanjun^a,b, GAO Jing^a, ZHOU Liya^a, HE Ying^a, JIA Fei^a

a Institute of Chemical Technology, Hebei University of Technology, Tianjin 300130, China;
b State Key Laboratory of Biology Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China

Received:2012-10-30 Revised:2012-12-24 Online:2013-04-23 Published:2013-04-24
Supported by:
This work was supported by the National Nature Science Foundation of China (21006020, 21276060, 21276062), the Natural Science Foundation of Hebei Province (B2010000035, B2011202095), the Science and Technology Research Key Project of Higher School in Hebei Province (ZD2010118) and the Application Basic Research Plan Key Basic Research Project of Hebei Province (11965150D), and the Open Funding Project of the National Key Laboratory of Biochemical Engineering (China).

摘要/Abstract

摘要：

将脂质体囊泡与仿生硅化技术相结合,模拟细胞纳微环境,实现以脂质体为模板仿生制备氧化硅固定葡萄糖氧化酶(GOx),建立性能稳定的固定化酶.扫描电镜分析显示,固定化GOx为球形纳米粒子,粒径分布在200nm左右,在优化反应条件下GOx回收率达到71.8%.由于载体的空间限制作用及其提供的较稳定微环境,固定化GOx表现出良好的热稳定性和pH稳定性,其对变性剂耐受性和操作稳定性等也得到明显提高.

关键词: 仿生硅化, 脂质体, 葡萄糖氧化酶, 固定化酶

Abstract:

Glucose oxidase (GOx) was immobilized in biomimetic silica particles by coupling liposome vesicles with a biomimetic silicification process simulating a cell microenvironment. SEM images showed that the immobilized GOx particles were spherical, and their diameter was approximately 200 nm. The recovery of GOx reached 71.8% under the optimized reaction conditions. The immobilized GOx showed improved thermal and pH stability, and its tolerance to denaturants and reusability were also greatly improved. This was attributed to the space limitations and stable environment in the support.

Key words: Biomimetic silicification, Liposome, Glucose oxidase, Immobilized enzyme

朱亚男, 姜艳军, 高静, 周丽亚, 贺莹, 贾霏. 脂质体为模板仿生硅化固定葡萄糖氧化酶[J]. 催化学报, 2013, 34(4): 741-750.

ZHU Yanan, JIANG Yanjun, GAO Jing, ZHOU Liya, HE Ying, JIA Fei. Immobilization of glucose oxidase in liposome-templated biomimetic silica particles[J]. Chinese Journal of Catalysis, 2013, 34(4): 741-750.

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参考文献

[1] de Marcos S, Galindo J, Sierra J F, Galban J, Castillo J R. Sens Actuators B, 1999, 57: 227

[2] Reetz M T, Tielmann P, Wiesenhöfer W, Könen W, Zonta A. Adv Synth Catal, 2003, 345: 717

[3] Keeling-Tucker T, Rakic M, Spong C, Brennan J D. Chem Mater, 2000, 12: 3695

[4] Jin W, Brennan J D. Anal Chim Acta, 2002, 461: 1

[5] Das S, Berke-Schlessel D, Ji H F, McDonough J, Wei Y. J Mol Catal B, 2011, 70: 49

[6] Campbell A, Taylor P, Cayre O J, Paunov V N. Chem Commun, 2004: 2378

[7] Nara T Y, Togashi H, Ono S, Egami M, Sekikawa C, Suzuki Y-h, Masuda I, Ogawa J, Horinouchi N, Shimizu S, Mizukami F, Tsunoda T. J Mol Catal B, 2011, 68: 181

[8] Ambati J, Lopez A M, Cochran D, Wattamwar P, Bean K, Dziubla T D, Rankin S E. Acta Biomater, 2012, 8: 2096

[9] Wang Y J, Caruso F. Chem Mater, 2006, 18: 4089

[10] Begu S, Girod S, Lerner D A, Jardiller N, Tourne-Peteilh C, Devoisselle J M. J Mater Chem, 2004, 14: 1316

[11] Steinberg Y, Schroeder A, Talmon Y, Schmidt J, Khalfin R L, Cohen Y, Devoisselle J M, Begu S, Avnir D. Langmuir, 2007, 23: 12024

[12] Li Y, Yip W T. J Am Chem Soc, 2005, 127: 12756

[13] Natalio F, Link T, Müller W E G, Schröder H C, Cui F Zh, Wang X H, Wiens M. Acta Biomater, 2010, 6: 3720

[14] Müller W E G, Boreiko A, Wang X H, Belikov S I, Wiens M, Grebenjuk V A, Schlossmacher U, Schröder H C. Gene, 2007, 395: 62

[15] Roth K M, Zhou Y, Yang W J, Morse D E. J Am Chem Soc, 2005, 127: 325

[16] Wiens M, Bausen M, Natalio F, Link T, Schlossmacher U, Müller W E G. Biomaterials, 2009, 30: 1648

[17] Galarneau A, Mureseanu M, Atger S, Renard G, Fajula F. New J Chem, 2006, 30: 562

[18] Galarneau A, Sartori F, Cangiotti M, Mineva T, Di Renzo F, Ottaviani M F. J Phys Chem B, 2010, 114: 2140

[19] Phuoc L T, Laveille P, Chamouleau F, Renard G, Drone J, Coq B, Fajula F, Galarneau A. Dalton Trans, 2010, 39: 8511

[20] Galarneau A, Renard G, Mureseanu M, Tourrette A, Biolley C, Choi M, Ryoo R, Di Renzo F, Fajula F. Microporous Mesoporous Mater, 2007, 104: 103

[21] Li Q W, Luo G A, Wang Y M, Zhang X R. Mater Sci Eng C, 2000, 11: 67

[22] Devaraj K B, Kumar P R, Prakash V. Process Biochem, 2011, 46: 458

[23] Taboada-Puig R, Junghanns C, Demarche P, Moreira M T, Feijoo G, Lema J M, Agathos S N. Bioresour Technol, 2011, 102: 6593

[24] Luckarift H R, Spain J C, Naik R R, Stone M O. Nat Biotechnol, 2004, 22: 211

脂质体为模板仿生硅化固定葡萄糖氧化酶

Immobilization of glucose oxidase in liposome-templated biomimetic silica particles

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