催化学报

催化学报 ›› 2009, Vol. 30 ›› Issue (5): 396-400.

• 研究论文 • 上一篇    下一篇

水解酶在二乙基锌与芳香醛加成反应中的应用

魏晓飞1, 郑清川2, 吉腾飞3, 赵博1, 李春元1, 王萍1, 曹淑桂1, 王智1, 王磊1   

  1. 1吉林大学分子酶学工程教育部重点实验室, 吉林长春130023 2吉林大学理论化学计算国家重点实验室, 吉林长春130023 3中国医学科学院药物研究所, 北京100050
  • 出版日期:2009-05-25 发布日期:2013-02-05

Addition of Diethylzinc to Aromatic Aldehydes Catalyzed by Hydrolase

WEI Xiaofei1, ZHENG Qingchuan2, JI Tengfei3, ZHAO Bo1, LI Chunyuan1, WANG Ping1, CAO Shugui1, WANG Zhi1, #, WANG Lei1, *   

  1. 1Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun 130023, Jilin, China 2State Key Laboratory of Theoretical and Computational Chemistry, Jilin University, Changchun 130023, Jilin, China 3Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, China
  • Online:2009-05-25 Published:2013-02-05

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824

被引次数

2

摘要： 报道了水解酶催化二乙基锌与芳香醛的加成反应, 对反应条件进行了优化. 在最适条件(嗜热酯酶APE1547为酶源, 反应温度40 oC, 氯仿为溶剂, 4-Cl-苯甲醛为底物)下, 加成反应生成的光学活性醇产率最高达78%, ee值最高可达56%. 通过实验结果和分子动力学分析对可能的反应机理进行了推测. 本研究进一步拓展了酶的非专一性.

关键词: 水解酶, 二乙基锌, 芳香醛, 加成, 酶, 非专一性

Abstract: A novel application of hydrolase as catalyst for the addition of diethylzinc to aromatic aldehydes is reported, and a possible mechanism is proposed based on experimental observations and molecular dynamics simulation. The present observations extend the phenomenon of enzyme promiscuity.

Key words: hydrolase, diethylzinc, aromatic aldehyde, addition, enzyme, promiscuity