催化学报

催化学报 ›› 2006, Vol. 27 ›› Issue (10): 875-879.

• 研究论文 • 上一篇    下一篇

谷胱甘肽过氧化物酶模拟物碲化透明质酸的合成及催化动力学研究

张博旬1,陈智博1,安洋3,彭清林1,陈佳1,张建国2,刘兰英1   

  1. 1 吉林大学生命科学学院, 吉林长春 130023; 2 中国科学院长春应用化学研究所电分析化学国家重点实验室, 吉林长春 130022; 3 吉林大学医院, 吉林长春 130021
  • 收稿日期:2006-10-25 出版日期:2006-10-25 发布日期:2010-11-28

Synthesis and Catalytic Kinetics of Tellurium-Modified Hyaluronic Acid with Glutathione Peroxidase Activity

ZHANG Boxun1, CHEN Zhibo1, AN Yang3, PENG Qinglin1, CHEN Jia1, ZHANG Jianguo2, LIU Lanying1*   

  1. 1 College of Life Science, Jilin University, Changchun 130023, Jilin, China; 2 State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, The Chinese Academy of Sciences, Changchun 130022, Jilin, China; 3 Hospital of Jilin University, Changchun 130021, Jilin, China
  • Received:2006-10-25 Online:2006-10-25 Published:2010-11-28

PDF

986

被引次数

6

摘要： 以透明质酸(HA)作为谷胱甘肽过氧化物酶(GPX)的酶模型,将碲(Te)引入HA中,合成了一种新型的高活力的GPX模拟物碲化透明质酸(TeHA). 用红外光谱和核磁共振技术对TeHA的结构进行了研究,证明Te的修饰位点位于HA的N-乙酰氨基葡萄糖的羟甲基(-CH2OH)上. 采用Wilson辅酶偶联法测定得到TeHA催化还原型谷胱甘肽(GSH)还原H2O2的GPX活力为163.6 U/μmol, 高于文献报道的其它模拟酶. TeHA还能够催化GSH还原异丙苯基过氧化物(CuOOH)和叔丁基过氧化物(t-BuOOH)的反应,并且CuOOH为该模拟酶的最适底物. 通过研究TeHA催化GSH还原三种不同过氧化物的反应动力学发现, TeHA的催化遵循乒乓机制.

关键词: 透明质酸, 碲, 谷胱甘肽过氧化物酶, 动力学

Abstract: A novel mimic TeHA was synthesized by modifying hyaluronic acid (HA) with tellurium, whose function is similar to that of glutathione peroxidase (GPX). The structure of TeHA was characterized by means of infrared spectroscopy and nuclear magnetic resonance spectroscopy, showing that the target Te is located at -CH2OH of the N-acetyl-D-glucosamine of HA. The activity of TeHA is 163.6 U/μmol according to Wilson′s method. In contrast to other mimics, TeHA displays a high activity. Moreover, TeHA can use many hydroperoxides as substrates, such as H2O2, cumenyl hydroperoxide, and tert-butyl hydroperoxide, and cumenyl hydroperoxide is the optimal substrate. A ping-pong mechanism was deduced for the reduction reactions catalyzed by TeHA according to the steady-state kinetic studies.

Key words: hyaluronic acid, tellurium, glutathione peroxidase, kinetics