游离脂肪酶 NS81006 催化油脂醇解制备生物柴油的酶促反应动力学

doi:10.3724/SP.J.1088.2012.20638

催化学报 ›› 2012, Vol. 33 ›› Issue (11): 1857-1861.DOI: 10.3724/SP.J.1088.2012.20638

游离脂肪酶 NS81006 催化油脂醇解制备生物柴油的酶促反应动力学

吕亮亮, 杜伟, 刘德华

清华大学化工系, 北京 100084

收稿日期:2012-07-20 修回日期:2012-09-17 出版日期:2012-11-16 发布日期:2012-11-16

Kinetics of Liquid Lipase NS81006-Catalyzed Alcoholysis of Oil for Biodiesel Production

LÜ Liangliang, DU Weia, LIU Dehuab

Department of Chemical Engineering, Tsinghua University, Beijing100084, China

Received:2012-07-20 Revised:2012-09-17 Online:2012-11-16 Published:2012-11-16

摘要/Abstract

摘要： 探究了游离脂肪酶 NS81006 催化油脂甲 (乙) 醇解制备生物柴油的反应历程, 并对该体系进行了酶促反应动力学研究. 结果表明, 催化过程中油脂同时存在酯交换及先水解再酯化两种反应历程. 在以甲醇或乙醇作为酰基受体的反应过程中, 酯交换反应速率明显大于水解反应速率. 进一步研究表明, 油脂甲醇解反应速率常数大于乙醇解, 揭示了以甲醇或乙醇为不同酰基受体时反应速率存在差异的主要原因在于乙醇解反应的酯交换过程较慢.

关键词: 脂肪酶, 生物柴油, 反应动力学, 酯交换反应, 水解反应

Abstract: Biodiesel production catalyzed by liquid lipase has been drawing more attention for its lower cost and faster reaction rate compared to immobilized lipase. The liquid lipase NS81006-catalyzed methanolysis and ethanolysis of soybean oil for biodiesel production were studied. A kinetic model was established by taking transesterification reactions, as well as hydrolysis and esterification reactions into consideration. The corresponding values of rate constants were then obtained for all forward and reverse reactions based on the model. The results show that the transesterification reaction rates are obviously higher than the hydrolysis reaction rates either with methanol or ethanol as the acyl acceptor. Further study indicates that the rate constants of ethanolysis are smaller than that of methanoslysis, which results in the difference in reaction rate between methanolysis and ethanolysis.

Key words: lipase, biodiesel, kinetics, transesterification, hydrolysis reaction

吕亮亮, 杜伟, 刘德华. 游离脂肪酶 NS81006 催化油脂醇解制备生物柴油的酶促反应动力学[J]. 催化学报, 2012, 33(11): 1857-1861.

Lü Liang-Liang, DU Wei, LIU De-Hua. Kinetics of Liquid Lipase NS81006-Catalyzed Alcoholysis of Oil for Biodiesel Production[J]. Chinese Journal of Catalysis, 2012, 33(11): 1857-1861.

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游离脂肪酶 NS81006 催化油脂醇解制备生物柴油的酶促反应动力学

Kinetics of Liquid Lipase NS81006-Catalyzed Alcoholysis of Oil for Biodiesel Production

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