Light-driven CO2 assimilation by photosystem II and its relation to photosynthesis

doi:10.1016/S1872-2067(22)64170-6

Abstract

Abstract:

For natural oxygenic photosynthesis, there is a consensus that H₂O is oxidized to molecular oxygen by photosystem II (PSII) in the grana, while CO₂ assimilation takes place, long after oxygen evolution, in light-independent reactions, for example, through the Calvin-Benson Cycle in the stroma. Here, we report, for the first time, light-driven CO₂ assimilation by the PSII core complex, where the formation of methanol, along with the oxygen evolution, is validated by in-situ mass spectrometry, gas chromatography and isotopic labeling experiments. Such an unusual CO₂ assimilation is likely to be a simultaneous event along with the usual electron transfer occurring in normal light-independent assimilation. This discovery is extraordinary and is of great significance as it may substantially modify our understanding of the mechanism of photosynthesis.

Key words: Photosynthesis, Photosystem II core complex, Photoreaction, CO₂ assimilation, Methanol

Yuehui Li, Duanhui Si, Wangyin Wang, Song Xue, Wenzhe Shang, Zhanyou Chi, Can Li, Ce Hao, Govindjee Govindjee, Yantao Shi. Light-driven CO₂ assimilation by photosystem II and its relation to photosynthesis[J]. Chinese Journal of Catalysis, 2023, 44: 117-126.

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URL: https://www.cjcatal.com/EN/10.1016/S1872-2067(22)64170-6

https://www.cjcatal.com/EN/Y2023/V44/I1/117

Figures/Tables 4

Fig. 1. The measurements of the production of O2 and CH3OH by the green alga Chlorella and chloroplasts, isolated from spinach leaves, upon light illumination. (a,b) A diagram of Chlorella cells and spinach chloroplasts. (c,d) The O2 and CH3OH detected by in-situ real-time MS in Chlorella and spinach suspensions. (e,f) The CH3OH detected by GC in Chlorella cells and in spinach chloroplasts. The blank experiments E and F refer to those carried out in the dark.

Fig. 2. The labeled CH3OH detected by in-situ RT-MS in Chlorella cells and spinach chloroplasts upon light illumination. (a,b) The 13CH3OH (m/z = 33) detected by RT-MS for Chlorella and spinach chloroplasts labeled with 13CO2. (c,d) The CH318OH (m/z = 33) detected by RT-MS for Chlorella and spinach chloroplasts, labeled with C18O2.

Fig. 3. The measurements of the production of O2 and CH3OH by the PSII core complex upon light illumination. (a) The O2 and CH3OH detected by in-situ RT-MS. (b) The CH3OH detected by GC; The O2 (c) and CH3OH (d) relative amounts detected by RT-MS under different concentration of CO2 in carrier gas (0, 88, 220, 1175, 10000, 15000 ppm). The 13CH3OH (e) and CH318OH (f) detected by in-situ RT-MS in 13CO2 and C18O2 labeling experiments.

Fig. 4. The O2 (a) and CH3OH (b) detected by RT-MS for the PSII core complex, upon light illumination, involving different electron accepters. (c) A schematic depiction of the electron flow across the three protein complexes, as well as the light-driven CO2 light assimilation possibly occurring prior to QA.

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Light-driven CO₂ assimilation by photosystem II and its relation to photosynthesis

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