催化学报

催化学报 ›› 2011, Vol. 32 ›› Issue (6): 1051-1055.DOI: 10.3724/SP.J.1088.2011.01211

• 研究论文 • 上一篇    下一篇

离子液体中 β-葡萄糖苷酶生物催化合成红景天甙

王梦亮*, 郭春侠   

  1. 山西大学应用化学研究所, 山西太原 030006
  • 收稿日期:2010-12-08 修回日期:2011-03-09 出版日期:2011-06-21 发布日期:2014-10-31

Biocatalytic Synthesis of Salidroside by β-Glucosidase in Ionic Liquids

WANG Mengliang*, GUO Chunxia   

  1. Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, Shanxi, China
  • Received:2010-12-08 Revised:2011-03-09 Online:2011-06-21 Published:2014-10-31

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摘要： 比较了聚乙二醇修饰的 β-葡萄糖苷酶在三种离子液体 ([bmim]PF6, [bmim]BF4, [bmim]Tf2N) 及两种常用有机溶剂 (1,4-二氧六环、正己烷) 中的催化性能, 确定了适宜的反应介质; 考察了底物浓度、温度、体系含水量和反应时间等因素对 β-葡萄糖苷酶催化合成红景天甙反应的影响. 结果表明, 在最佳反应介质[bmim]PF6 存在下, 底物酪醇浓度为 70 mmol/L, 体系含水量 2%, 于 50 oC, 反应 24 h 时, 高达 88.5% 的酪醇转化为红景天甙 (即 61.93 mmo1/L). 与有机溶剂相比, 以[bmim]PF6 为反应介质对酶促合成红景天甙的反应过程具有明显的强化作用.

关键词: 离子液体, β-葡萄糖苷酶, 红景天甙, 生物催化, 酪醇

Abstract: β-Glucosidase modified with polyethylene glycol (PEG) was used as catalyst for the synthesis of salidroside from tyrosol and D-glucose, and its catalytic activity in three ionic liquids, [bmim]PF6, [bmim]BF4, and [bmim]Tf2N, and two common organic solvents, 1,4-dioxane and n-hexane, was measured. Ionic liquid [bmim]PF6 was the optimal reaction medium, and different reaction conditions were optimized in it. A yield of 88.5% (product content of 61.93 mmo1/L) was obtained under the conditions of H2O content of 2%, temperature of 50 oC, tyrosol content of 70 mmo1/L, D-glucose/tyrosol molar ratio of 2, and reaction time of 24 h. The results suggest that both the modification with PEG and using ionic liquid [bmim]PF6 as reaction medium have obvious promoting effects on the enzymatic synthesis of salidroside.

Key words: ionic liquid, β-glucosidase, salidroside, biocatalysis, tyrosol