催化学报

催化学报 ›› 2012, Vol. 33 ›› Issue (7): 1161-1165.DOI: 10.1016/S1872-2067(11)60395-1

• 研究论文 • 上一篇    下一篇

离子液体对 β-糖苷酶催化合成红景天苷的影响

毕艳红, 王朝宇*, 茅燕勇, 郑尚永, 张海江, 时号   

  1. 淮阴工学院生命科学与化学工程学院, 江苏淮安 223003
  • 收稿日期:2012-02-23 修回日期:2012-04-13 出版日期:2012-06-21 发布日期:2012-06-21

Ionic Liquid Effects on the Activity of β-Glycosidase for the Synthesis of Salidroside in Co-solvent Systems

BI Yanhong, WANG Zhaoyu*, MAO Yanyong, ZHENG Shangyong, ZHANG Haijiang, SHI Hao   

  1. School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huai’an 223003, Jiangsu, China
  • Received:2012-02-23 Revised:2012-04-13 Online:2012-06-21 Published:2012-06-21

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摘要： 研究了混合溶剂体系中阴离子分别为 BF4-, PF6-, Cl-, Br-和I-的 14 种离子液体对来源于黑布林糖苷酶催化合成红景天苷反应的影响. 结果表明, 在最佳反应条件下, β-糖苷酶的反应初速度和红景天苷的产率分别为 3.3 mmol/(L•h) 和 24.5%. 离子液体咪唑阳离子的烷基链长 (C2~C10) 对 β-糖苷酶的活性影响较大, 当烷基链长为 C6 时, 糖苷酶表现出较高的催化活性.

关键词: 离子液体, 红景天苷, 糖苷化反应, 黑布林籽, 混合溶剂

Abstract: The preparation of salidroside was successfully carried out in fourteen ionic liquids (ILs)-containing systems using β-glycosidase from black plum seeds for the first time. The optimum conditions were determined for C6MIm?BF4, pH, phosphate buffer content, and molar ratio of tyrosol to D-glucose to be 1% (v/v), 5.9, 20% (v/v), and 8:1, under which the initial reaction rate and yield were 3.3 mmol/(L·h) and 24.5%, respectively. Moreover, the effects of 1-alkylimidazolium-based ILs possessing different alkyl chain lengths from C2 to C10 and a variety of anions including BF4-, PF6-, Cl-, Br-, and I- on enzyme activity in co-solvent systems were investigated. The results indicate that the optimal chain length of the alkyl substituent on the imidazolium ring of the cation was C6.

Key words: ionic liquid, salidroside, glucosylation, black plum seed meal, co-solvent