均相介质中脂肪酶催化沙丁鱼油甘油解反应的动力学研究

doi:10.1016/S1872-2067(15)61040-3

催化学报 ›› 2016, Vol. 37 ›› Issue (4): 596-606.DOI: 10.1016/S1872-2067(15)61040-3

均相介质中脂肪酶催化沙丁鱼油甘油解反应的动力学研究

Ángela García Solaesa, María Teresa Sanz, Sagrario Beltrán, Rodrigo Melgosa

布尔戈斯大学生物技术与食品科学系(化学工程部), 布尔戈斯 09001, 西班牙

收稿日期:2015-11-18 修回日期:2016-01-04 出版日期:2016-03-30 发布日期:2016-03-30
通讯作者: María Teresa Sanz

Kinetic study and kinetic parameters of lipase-catalyzed glycerolysis of sardine oil in a homogeneous medium

Ángela García Solaesa, María Teresa Sanz, Sagrario Beltrán, Rodrigo Melgosa

Department of Biotechnology and Food Science (Chemical Engineering Section), University of Burgos, Burgos 09001, Spain

Received:2015-11-18 Revised:2016-01-04 Online:2016-03-30 Published:2016-03-30
Contact: María Teresa Sanz

摘要/Abstract

摘要：

多不饱和脂肪酸(PUFAs)的生产多集中在酶催化, 与无机催化相比, 酶催化具有定向性和更温和的反应条件, 因而酶催化制取多不饱和脂肪酸的路线在最近几年引起人们极大的兴趣. 本文以优化量的叔丁醇为有机溶剂, 研究了脂肪酶Lipozyme® 435 催化沙丁鱼油甘油解反应生成 PUFA (多以甘油一酯或二酯(DAGs, MAGs)的形式存在). 首先分析了反应系统的传质影响, 考察了脂肪酶载量、温度和进料浓度等因素的影响. 采用一个基于甘油酯甘油解和水解这对可逆基元反应的半经验动力学模型, 成功地关联了实验的动力学数据. 结果表明, 温度为 323 K, 甘油与油的摩尔比为 3:1 时, 生成的 MAG 可达 84 wt%以上. 还考察了其他甘油解体系, 并在 MAG 收率、反应速率和动力学参数意义等方面进行了比较.

关键词: 脂肪酶, 甘油解反应, 叔丁醇, 传质, 动力学模型

Abstract:

The production of polyunsaturated fatty acids (PUFAs) concentrates by enzymatic catalysis has gained interest due to their stereospecificity and the milder conditions employed compared to the use of inorganic catalysts. The enzymatic glycerolysis of sardine oil by Lipozyme® 435 to get PUFA concentrates in the forms of di- and monoacylglycerols (DAGs, MAGs) in an optimized amount of tert-butanol as the organic solvent was studied. First, mass transfer limitation of the reaction system was analyzed. The effects of different operating variables such as lipase loading, temperature and feed composition were investigated. A semi-empirical kinetic model based on the reversible elementary reactions of glycerolysis and hydrolysis of the glycerides was employed to correlate the experimental kinetic data. A molar ratio glycerol:oil of 3:1 was the optimum, which produced more than 84 wt% of MAG at 323 K. A comparison with other glycerolysis systems was performed using MAG yield, reaction rate and significance of kinetic parameters.

Key words: Lipase, Glycerolysis, Tert-butanol, Mass transfer, Kinetic model

Ángela García Solaesa, María Teresa Sanz, Sagrario Beltrán, Rodrigo Melgosa. 均相介质中脂肪酶催化沙丁鱼油甘油解反应的动力学研究[J]. 催化学报, 2016, 37(4): 596-606.

Ángela García Solaesa, María Teresa Sanz, Sagrario Beltrán, Rodrigo Melgosa. Kinetic study and kinetic parameters of lipase-catalyzed glycerolysis of sardine oil in a homogeneous medium[J]. Chinese Journal of Catalysis, 2016, 37(4): 596-606.

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均相介质中脂肪酶催化沙丁鱼油甘油解反应的动力学研究

Kinetic study and kinetic parameters of lipase-catalyzed glycerolysis of sardine oil in a homogeneous medium

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